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Nicotine-induced chondrogenic differentiation of human bone marrow stromal cells in vitro

  • Experimental Study
  • Published:
Knee Surgery, Sports Traumatology, Arthroscopy Aims and scope

Abstract

Purpose

Nicotine has been reported that it has a dose-dependent effect on matrix mineralization by human bone marrow cells. However, there is no relevant research concerning on chondrogenic differentiation potential of bone marrow stromal stem cells (BMSCs) treated with nicotine in vitro. The aims of the study were to examine the effects of nicotine (0, 10−7, 10−6 and 10−5 M) on the proliferation and chondrogenic differentiation of BMSCs from three healthy donors in vitro.

Methods

BMSCs proliferation was analyzed by CCK8 assay and real-time polymerase chain reaction was used to assay the expression of type II collagen, aggrecan, type I collagen and type X collagen. The proteoglycan content was stained by Alcian blue, and the sulfated glycosaminoglycan (sGAG) content of BMSCs was quantified spectrofluorometrically using dimethylmethylene blue.

Results

The cell viability was not significantly impaired until up to a concentration of 10−5 M nicotine. Nicotine promoted the proliferation and enhanced the expression of type II collagen at the level up to 10−6 M (P < 0.05). The expression of aggrecan was reduced at the concentration of 10−5 M nicotine at day 14 (P < 0.05), and there was no significant difference in aggrecan gene expression at 10−7 and 10−6 M nicotine levels compared to control group (n.s.). Also the fibroblastic and hypertrophic gene expressions were down-regulated in the chondrogenic medium with 10−7–10−5 M nicotine (P < 0.05).

Conclusion

It was implied that local application of nicotine at an appropriate concentration may be a promising approach for enhancing chondrogenic differentiation capacity of BMSCs in cell-based cartilage tissue engineering. Also these results indicate that nicotine maybe a potentially useful drug for the treatment of Osteoarthritis.

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Acknowledgments

The authors thank all the staff in the Laboratory of Orthopaedic Research Institute and Scientific Research Center of Second Affiliated Hospital of Wenzhou Medical College. This work was supported by grants from the National Natural Science Foundation of China (31010121), Hainan Provincial Health Department (2011-34), Medicines and Health Research fund of Zhejiang (2007B147), Zhejiang Science and Technology Project (2010C34G2090013), Wenzhou Technology Project (Y20090288), Wenzhou Technology Project (Y20070133).

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Authors and Affiliations

  1. Department of Orthopaedic Surgery, The Second Affiliated Hospital of Wenzhou Medical College, 109 Xue Yuan Xi Road, Wenzhou, China

    Xiaozhou Ying, Wei Zhang, Shaowen Cheng, Pengfei Nie, Xiaojie Cheng, Yue Shen, Wei Wang, Enxing Xue, Qingyu Chen, Dongquan Kou, Lei Peng & Yu Zhang

  2. Trauma Center of the Affiliated Hospital of Hainan Medical College, 31 Long Hua Road, Haikou, 571100, China

    Lei Peng & Chuanzhu Lu

  3. The Second Affiliated Hospital of Wenzhou Medical College; Trauma Center of the Affiliated Hospital of Hainan Medical College, 109 Xue Yuan Xi Road, Wenzhou, 325000, China

    Lei Peng

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  1. Xiaozhou Ying
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  2. Wei Zhang
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  3. Shaowen Cheng
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  7. Wei Wang
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  11. Lei Peng
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Correspondence to Lei Peng, Yu Zhang or Chuanzhu Lu.

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Ying, X., Zhang, W., Cheng, S. et al. Nicotine-induced chondrogenic differentiation of human bone marrow stromal cells in vitro. Knee Surg Sports Traumatol Arthrosc 20, 2329–2336 (2012). https://doi.org/10.1007/s00167-012-1890-0

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  • DOI: https://doi.org/10.1007/s00167-012-1890-0

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