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Design and evaluation of a recombinant multi-epitope-based ELISA for the serological surveillance of HEV infection in northern China

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Abstract

In this study, seven recombinant epitope peptides from within the ORF2 protein of the local genotype 4 swine hepatitis E virus (HEV) DQ strain were designed and analyzed. Then, a new multi-epitope-based ELISA was established. In comparison with a commercial kit, this test exhibited good specificity and sensitivity for anti-HEV genotype 4. Subsequently, this test was applied for analyzing serum samples from either swine herds or human populations in northern China. The overall seroprevalence rate of anti-HEV IgG reached up to 40.4% for swine and 8.1% for humans. A statistical difference was observed for humans in rural and urban areas, with a higher prevalence for people living in rural than urban areas. Moreover, sequencing confirmed that all RNA-positive samples belonged to genotype 4.

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Acknowledgments

The authors wish to thank Dr. Xiang WH. for his technical assistance. This work was financed by a grant from Liaoning, China.

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Authors and Affiliations

  1. Department of Animal Husbandry & Veterinary Medicine, Liaoning Medical University, Renmin Street 5-48, Jinzhou, 121001, China

    Gao Shenyang & Zhou Tiezhong

  2. Animal Quarantine Lab, Inspection & Quarantine Technology Center of Hainan Entry-Exit Inspection & Quarantine Bureau, Haikou, 570000, China

    Li Dandan & Wu Shuliang

  3. Agricultural Department, Liaocheng University, Liaocheng, 252000, China

    Fan Chen

Authors
  1. Gao Shenyang
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  2. Li Dandan
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  3. Fan Chen
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  4. Wu Shuliang
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  5. Zhou Tiezhong
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Corresponding author

Correspondence to Zhou Tiezhong.

Additional information

G. Shenyang and L. Dandan equally contributed.

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Shenyang, G., Dandan, L., Chen, F. et al. Design and evaluation of a recombinant multi-epitope-based ELISA for the serological surveillance of HEV infection in northern China. Arch Virol 156, 1621–1626 (2011). https://doi.org/10.1007/s00705-011-1007-y

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  • DOI: https://doi.org/10.1007/s00705-011-1007-y

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