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Genetic diversity and phylogenetic relationship of Tadehagi in southwest China evaluated by inter-simple sequence repeat (ISSR)

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Abstract

There are many valuable Tadehagi accessions in southwest China, but it is unknown that the genetic diversity and phylogenetic relationship of these Tadehagi resources. This report is the first study in which 41 primers of inter-simple sequence repeat (ISSR) were used to assess the genetic diversity of 36 Tadehagi accessions from 3 provinces in the southwest of China. Totally, 30 usable ISSR primers detected 163 polymorphic bands among the 36 accessions, which suggested high utility of ISSR primers in the genetic analysis of Tadehagi accessions. Genetic similarity coefficients among all of the accessions ranged from 0.54 to 0.92 with the average of 0.79 based on the ISSR data, indicating high level of genetic variation in Tadehagi resources from the southwest of China. As for the 3 population, Hainan population had the maximum average genetic similarity coefficients of 0.81, while similarity coefficient of Guangxi and Yunnan population was 0.75 and 0.74, respectively. All the 36 Tadehagi accessions were divided into 4 groups in the UPGMA dendrogram constructed from genetic similarity coefficients. The Tadehagi accessions from Yunnan and Guangxi provinces showed more genetic variation and occupied the bottom of the dendrogram. On the contrary, those from Hainan Province had less genetic variation and clustered in the middle and top of the dendrogram. The information on the genetic diversity and phylogenetic relationship from this study is propitious to construct a core germplasm collection and develop novel Tadehagi cultivars with desired economic traits.

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Acknowledgments

Financial support of this research from the Yunnan Provincial Natural Science Foundation (2008ZC036 M) was appreciated.

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Authors and Affiliations

  1. Faculty of Life Science and Technology, Kunming University of Science and Technology, Yunnan, 650224, China

    Diqiu Liu

  2. Yunnan Beef Cattle & Pasture Research Centre, Kunming, Yunnan, 650212, China

    Xin He & Bizhi Huang

  3. China Institute of Tropical Crop Genetic Resources, Chinese Academy of Tropical Agricultural Sciences, Danzhou, Hainan, 571737, China

    Guodao Liu

Authors
  1. Diqiu Liu
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  2. Xin He
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  3. Guodao Liu
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  4. Bizhi Huang
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Correspondence to Bizhi Huang.

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Diqiu Liu and Xin He contributed equally to this work.

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Liu, D., He, X., Liu, G. et al. Genetic diversity and phylogenetic relationship of Tadehagi in southwest China evaluated by inter-simple sequence repeat (ISSR). Genet Resour Crop Evol 58, 679–688 (2011). https://doi.org/10.1007/s10722-010-9611-3

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  • DOI: https://doi.org/10.1007/s10722-010-9611-3

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