Skip to main content

Advertisement

Log in

Development and characterization of 27 SNP markers in the Mauremys mutica transcriptome

  • Technical Note
  • Published:
Conservation Genetics Resources Aims and scope Submit manuscript

Abstract

Asian yellow pond turtle (Mauremys mutica) is listed as an endangered species on the IUCN Red List and recorded in the CITES Appendix II. The wild populations of M. mutica are extremely rare in China, while the main living resources are captive populations. The development of the molecular markers is very important for population identification, population conservation and scientific management of M. mutica. In this study, 27 novel SNP markers for M. mutica were discovered and validated. The observed and expected heterozygosities ranged from 0.0361 to 0.9277 and 0.0588 to 0.5004, respectively. Seven loci showed significant deviation from HWE and no significant linkage disequilibrium was found. These markers would be used in genetic studies helping conservation and management of M. mutica.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

References

  • Gong SP, Chow AT, Fong JJ, Shi HT (2009) The chelonian trade in the largest pet market in China: scale, scope and impact on turtle conservation. Oryx 43(02):213–216

    Article  Google Scholar 

  • Hauser L, Baird M, Hilborn RAY et al (2011) An empirical comparison of SNPs and microsatellites for parentage and kinship assignment in a wild sockeye salmon (Oncorhynchus nerka) population. Mol Ecol Resour 11(s1):150–161

    Article  PubMed  Google Scholar 

  • Houston RD, Taggart JB, Cézard T et al (2014) Development and validation of a high density SNP genotyping array for Atlantic salmon (Salmo salar). BMC Genomics 15(1):90. doi:10.1186/1471-2164-15-90

    Article  CAS  Google Scholar 

  • Jiang L, Zhu A, Zhang J et al (2015) Development of forty-two single-nucleotide polymorphism markers in large yellow croaker (Larimichthys crocea). J Genet 94(2):1–4

    Google Scholar 

  • Kaiser SA, Taylor SA, Chen N et al (2017) A comparative assessment of SNP and microsatellite markers for assigning parentage in a socially monogamous bird. Mol Ecol Resour 17(2):183–193

    Article  CAS  PubMed  Google Scholar 

  • Kwong QB, Teh CK, Ong AL et al (2016) Development and validation of a high-density SNP genotyping array for African oil palm. Mol Plant 9(8):1132–1141. doi:10.1016/j.molp.2016.04.010

    Article  CAS  Google Scholar 

  • Small MP, Rogers Olive SD, Seeb LW et al (2015) Chum salmon genetic diversity in the northeastern Pacific Ocean assessed with single nucleotide polymorphisms (SNPs): applications to fishery management. North Am J Fish Manag 35(5):974–987

    Article  Google Scholar 

  • Vignal A, Milan D, SanCristobal M et al (2002) A review on SNP and other types of molecular markers and their use in animal genetics. Genet Sel Evol 34(3):275

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Yeh FC, Yang RC, Boyle T (2000). POPGENE ver 1.32, Microsoft Windows-based freeware for population genetic analysis, University of Alberta and the Centre for International Forestry Research

  • Yu PH, Yang PY, Chiu YS et al (2013) Hematologic and plasma biochemical reference values of the yellow pond turtle Mauremys mutica, and the effects of sex and season. Zool Stud 52(1):24

    Article  Google Scholar 

  • Zhang Y, Zhang JY, Zheng RQ (2010). Isolation and characterization of 14 polymorphic microsatellite loci in the Asian yellow pond turtle, Mauremys mutica (Cantor, 1842). Aquacult Res 41(9):e353–e356

    Article  CAS  Google Scholar 

  • Zhao J, Li W, Wen P, Zhang D et al (2016a) Genetic diversity and relationship of Mauremys mutica and M. annamensis assessed by dna barcoding sequences. Mitochondrial DNA A DNA Mapp Seq Anal 27(5):3507

    CAS  PubMed  Google Scholar 

  • Zhao J, Zhang X, Li W et al (2016b) SNP discovery and Characterization from transcriptomes of Asian yellow pond turtle, Mauremys mutica. Conserv Genet Resour 8(1):17–21

    Article  Google Scholar 

  • Zhu XP, Zhou L, Chen YL et al (2008) Phenotypic and genetic variation between two populations of the Chinese yellow pond turtle, Mauremys mutica (Cantor, 1842). High Technol Lett 14:104–111

    CAS  Google Scholar 

Download references

Acknowledgements

This research was funded by the National Natural Science Foundation of China (31302176).

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Zhu Xin-Ping.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Xin-Cheng, Z., Jian, Z., Wei, L. et al. Development and characterization of 27 SNP markers in the Mauremys mutica transcriptome. Conservation Genet Resour 10, 667–670 (2018). https://doi.org/10.1007/s12686-017-0897-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12686-017-0897-8

Keywords

Navigation