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Discoidin domain receptor 1 (DDR1), a promising biomarker, induces epithelial to mesenchymal transition in renal cancer cells

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Tumor Biology

Abstract

Discoidin domain receptor I (DDR1) is confirmed as a receptor tyrosine kinase (RTK), which plays a consequential role in a variety of cancers. Nevertheless, the influence of DDR1 expression and development in renal clear cell carcinoma (RCCC) are still not well corroborated. In our research, we firstly discovered that the expression level of DDR1 was remarkable related to TNM stage (p = 0.032), depth of tumor invasion (p = 0.047), and lymph node metastasis (p = 0.034) in 119 RCCC tissue samples using tissue microarray. The function of DDR1 was then evaluated in vitro using collagen I and DDR1 small interfering RNA (siRNA) to regulate the expression of DDR1 in OS-RC-2 and ACHN renal cancer cells (RCC). DDR1 expression correlated with increased RCC cell migration, invasion, and angiogenesis. Further study revealed that high expression of DDR1 can result in epithelial to mesenchymal transition (EMT) activation. Western blot assay showed that the N-cadherin protein and vimentin were induced while E-cadherin was reduced after DDR1 over expression. Our results suggest that DDR1 is both a prognostic marker for RCCC and a potential functional target for therapy.

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Acknowledgments

This study is supported by National Natural Science Foundation of China (NSFC), No.81301806; Jiangsu Planned Projects for Postdoctoral Research Funds, No.1501060A; six talent peaks project in Jiangsu Province, No.WSW-056.

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    Authors and Affiliations

    1. Jiangsu Key Laboratory of Biological Cancer Therapy, Xuzhou Medical College, Xuzhou, Jiangsu, 221002, China

      Jingyuan Song, Xiao Chen, Jin Bai, Qinghua Liu, Hui Li, Jianwan Xie, Hui Jing & Junnian Zheng

    2. Jiangsu Center for the Collaboration and Innovation of Cancer Biotherapy, Xuzhou, Jiangsu, 221002, China

      Junnian Zheng

    3. Affiliated Hospital of Xuzhou Medical College, Xuzhou, Jiangsu, 221002, China

      Junnian Zheng

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    1. Jingyuan Song
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    Jingyuan Song and Xiao Chen contributed equally to this work.

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    Song, J., Chen, X., Bai, J. et al. Discoidin domain receptor 1 (DDR1), a promising biomarker, induces epithelial to mesenchymal transition in renal cancer cells. Tumor Biol. 37, 11509–11521 (2016). https://doi.org/10.1007/s13277-016-5021-2

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