Determination and separation of putrescine and spermidine in aquatic products†
Abstract
A sensitive, simple and rapid CE-ECL method is established for the determination of putrescine and spermidine in aquatic products with a new luminous source material, PMo12–Ru(bpy)32+. This method is based on the electrochemical reaction of putrescine, spermidine and PMo12–Ru(bpy)32+ on a platinum electrode. The experimental conditions for the separation and detection of biogenic amines were optimized. Under optimal conditions, the detection limit (S/N = 3) was 6.5 × 10−3 mg L−1. For spermidine, the detection limit (S/N = 3) was 9.8 × 10−3 mg L−1. No spermidine was detected in the fresh ray samples while the content of putrescine was 0.019 mg L−1 in the samples. The relative standard deviation (RSD) of the peak height and migration time for putrescine was 1.19% and 1.11%, respectively, with a recovery of 90.0–104.0%. The new luminous source could make the CE-ECL method cleaner and more effective on the premise of guaranteeing accuracy.
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