Skip to main content

Advertisement

SpringerLink
Log in

Association of melamine exposure with urinary stone and oxidative DNA damage in infants

  • Organ Toxicity and Mechanisms
  • Published:
Archives of Toxicology Aims and scope Submit manuscript

Abstract

There is evidence in experimental animals for the urolithiasis and carcinogenicity of melamine, but no evidence for melamine in humans. To evaluate any association between melamine-contaminated powdered formula (MCPF) feeding and urolithiasis, and further the MCPF feeding and oxidative damage to DNA in infants. A cross-sectional study was carried out to assess urolithiasis and urinary 8-hydroxy-2′-deoxyguanosine (8-OHdG) in four groups of infants according to the type of feeding: (1) Infants receiving over 90% of their intake as MCPF. (2) Infants receiving 50–90% of their intake as MCPF. (3) Infants receiving less than 50% of their intake as MCPF. (4) Infants receiving over 90% of their intake as imported milk powdered formula free of melamine contamination. Groups 1 to 3 are the observation groups, and Group 4 is the reference group. There is a significant correlation between urolithiasis and percentage of MCPF intake. The mean urinary 8-OHdG concentrations for Groups 1, 2, 3, and 4 were: 2.03 ± 0.52, 1.67 ± 0.28, 1.90 ± 0.39, and 1.85 ± 0.47 micromoles per mole of creatinine, respectively. There were no significant differences in the mean urinary 8-OHdG concentrations among the observation and control groups. There were also no correlation between mean urinary 8-OHdG excretions and percentage of MCPF intake. Our data suggested that melamine exposure were associated with urolithiasis, but it might not cause any increase in oxidative damage of DNA in infants.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

Abbreviations

MCPF:

Melamine-contaminated powdered formula

8-OHdG:

8-Hydroxy-2′-deoxyguanosine

ROS:

Reactive oxygen species

IARC:

The International Agency for Research on Cancer

References

  • Barzilai A, Yamamoto K (2004) DNA damage responses to oxidative stress. DNA Repair 3:1109–1115

    Article  CAS  PubMed  Google Scholar 

  • Bowerman B (2005) Oxidative stress and cancer. Science 308:1119–1121

    Article  CAS  PubMed  Google Scholar 

  • Burgess JL, Meza MM, Josyula AB, Poplin GS, Kopplin MJ, McClellen HE, Strup S, Clark Lantz R (2007) Environmental arsenic exposure and urinary 8-OHdG in Arizona and Sonora. Clin Toxicol 45:490–498

    Article  CAS  Google Scholar 

  • Buthbumrung N, Mahidol C, Navasumrit P (2008) Oxidative DNA damage and influence of genetic polymorphisms among urban and rural schoolchildren exposed to benzene. Chem Biol Interact 172:185–194

    Article  CAS  PubMed  Google Scholar 

  • CHINADAILY (2008) http://bbs.chinadaily.com.cn/viewthread.php?gid=2&tid=615705&extra=&page=7. Updated October 2008

  • Chung CJ, Huang CJ, Pu YS, Su CT, Huang YK, Chen YT, Hsueh YM (2008) Urinary 8-hydroxydeoxyguanosine and urothelial carcinoma risk in low arsenic exposure area. Toxicol Appl Pharmacol 226:14–21

    Article  CAS  PubMed  Google Scholar 

  • David SS, Shea VL, Kundu S (2007) Base-excision repair of oxidative DNA damage. Nature 447:941–950

    Article  CAS  PubMed  Google Scholar 

  • Evans MD, Dizdaroglu M, Cooke MS (2004) Oxidative DNA damage and disease: induction, repair and significance. Mutat Res 567:1–61

    Article  CAS  PubMed  Google Scholar 

  • Guan N, Fan Q, Ding J, Ding J, Zhao Y, Lu J, Ai Y, Xu G, Zhu S, Yao C, Jiang L, Miao J, Zhang H, Zhao D, Liu X, Yao Y (2009) Melamine-contaminated powdered formula and urolithiasis in young children. N Engl J Med 360:1067–1074

    Article  CAS  PubMed  Google Scholar 

  • Irie M, Tamae K, Iwamoto-Tanaka N, Kasai H (2005) Occupational and lifestyle factors and urinary 8-hydroxydeoxyguanosine. Cancer Sci 96:600–606

    Article  CAS  PubMed  Google Scholar 

  • Karahalil B, Kocabas NA (2005) hOGG1 SER326CYS genetic polymorphism in a Turkish population. Arch Toxicol 79:377–380

    Article  PubMed  CAS  Google Scholar 

  • Kasai H, Svoboda P, Yamasaki S, Kawai K (2005) Simultaneous determination of 8-hydroxydeoxyguanosine, a marker of oxidative stress, and creatinine, a standardization compound, in urine. Ind Health 43:333–336

    Article  CAS  PubMed  Google Scholar 

  • Ke Y, Zhang Z, Jiang Y, Zhuang Z, Li L, Lu W, Wu T (2009) Association of hOGG1 genotype with life style and oxidative DNA damage among Chinese ethnic populations. Arch Toxicol 83:663–668

    Article  CAS  PubMed  Google Scholar 

  • Khan N, Sultana S (2005) Inhibition of potassium bromate-induced renal oxidative stress and hyperproliferative response by Nymphaea alba in Wistar rats. J Enzym Inhib Med Chem 20:275–283

    Article  CAS  Google Scholar 

  • Kristenson M, Kucinskiené Z, Schafer-Elinder L, Leandson P, Tagesson C (2003) Lower serum levels of beta-carotene in Lithuanian men are accompanied by higher urinary excretion of the oxidative DNA adduct, 8-hydroxydeoxyguanosine. Nutrition 19:11–15

    Article  CAS  PubMed  Google Scholar 

  • Liu AL, Lu WQ, Wang ZZ, Chen WH, Chen WH (2006) Elevated levels of urinary 8-hydroxy-2′-deoxyguanosine, lymphocytic micronuclei, and serum glutathione S-transferase in workers exposed to coke oven emissions. Environ Health Perspect 114:673–677

    Article  CAS  PubMed  Google Scholar 

  • Loft S, Vistisen K, Ewertz M, Tjønneland A, Overvad K, Poulsen HE (1992) Oxidative DNA damage estimated by 8-hydroxydeoxyguanosine excretion in humans: influence of smoking, gender and body mass index. Carcinogenesis 13:2241–2247

    Article  CAS  PubMed  Google Scholar 

  • Martino F, Loffredo L, Carnevale R, Sanguigni V, Martino E, Catasca E, Zanoni C, Pignatelli P, Violi F (2008) Oxidative stress is associated with arterial dysfunction and enhanced intima-media thickness in children with hypercholesterolemia: the potential role of nicotinamide-adenine dinucleotide phosphate oxidase. Pediatrics 122:e648–e655

    Article  PubMed  Google Scholar 

  • Meimaridou E, Lobos E, Hothersall JS (2006) Renal oxidative vulnerability due to changes in mitochondrial-glutathione and energy homeostasis in a rat model of calcium oxalate urolithiasis. Am J Physiol Renal Physiol 291:731–740

    Article  CAS  Google Scholar 

  • Moriyama MT, Suga K, Miyazawa K, Tanaka T, Higashioka M, Noda K, Oka M, Tanaka M, Suzuki K (2009) Inhibitions of urinary oxidative stress and renal calcium level by an extract of Quercus salicina Blume/Quercus stenophylla Makino in a rat calcium oxalate urolithiasis model. Int J Urol 16:397–401

    Article  CAS  PubMed  Google Scholar 

  • Puschner B, Poppenga RH, Lowenstine LJ, Filigenzi MS, Pesavento PA (2007) Assessment of melamine and cyanuric acid toxicity in cats. J Vet Diagn Invest 19:616–624

    PubMed  Google Scholar 

  • Schwille PO, Manoharan M, Schmiedl A (2005) Is idiopathic recurrent calcium urolithiasis in males a cellular disease? Laboratory findings in plasma, urine and erythrocytes, emphasizing the absence and presence of stones, oxidative and mineral. Clin Chem Lab Med 43:590–600

    Article  CAS  PubMed  Google Scholar 

  • Storz P (2005) Reactive oxygen species in tumor progression. Front Biosci 10:1881–1896

    Article  CAS  PubMed  Google Scholar 

  • Tagesson C, Kallberg M, Wingren G (1996) Urinary malondialdehyde and 8-hydroxydeoxyguanosine as potential markers of oxidative stress in industrial art glass workers. Int Arch Occup Environ Health 69:5–13

    Article  CAS  PubMed  Google Scholar 

  • Thompson E, Lewin-Smith MR, Kalasinsky VF, Pizzolato KM, Fleetwood ML, McElhaney MR, Johnson TO (2008) Characterization of melamine-containing and calcium oxalate crystals in three dogs with suspected pet food-induced nephrotoxicosis. Vet Pathol 45:417–426

    Article  CAS  PubMed  Google Scholar 

  • Tondel M, Arynchyn A, Jönsson P, Persson B, Tagesson C (2005) Urinary 8-hydroxydeoxyguanosine in Belarussian children relates to urban living rather than radiation dose after the chernobyl accident: a pilot study. Arch Environ Contam Toxicol 48:515–519

    Article  CAS  PubMed  Google Scholar 

  • WHO (2008) http://www.who.int/foodsafety/fs_management/infosan_events/en/index.html. Updated October 2008

  • Wong RH, Hu CW, Yeh CY, Chao MR, Chen CC, Huang JH, Chang SH, Lee SI, Lee HS (2008) Sulfotransferase 1A1 and glutathione S-transferase P1 genetic polymorphisms modulate the levels of urinary 8-hydroxy-2′-deoxyguanosine in betel quid chewers. Arch Toxicol 82:313–321

    Article  CAS  PubMed  Google Scholar 

  • Yu F, Liao Y, Zhao Y, Ren Y, Lu C, Li G, Li Y, Yang J (2008) Effects of in utero meso-2, 3-dimercaptosuccinic acid with calcium and ascorbic acid on lead-induced fetal development. Arch Toxicol 82:453–459

    Article  CAS  PubMed  Google Scholar 

  • Yuan J, Liu H, Zhou LH, Zou YL, Lu WQ (2006) Oxidative stress and DNA damage induced by a drinking-water chlorination disinfection byproduct 3-chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone (MX) in mice. Mutat Res 609:129–136

    CAS  PubMed  Google Scholar 

Download references

Acknowledgments

The research was supported by funds from the National Natural Science Foundation of China (30872149/C1504) and the Guangdong Natural Science Foundation (8451802003000336). We are grateful to Dr. Mak Ying-Tat from the Chinese University of Hong Kong, Hong Kong, for his revision. This study was conducted with assistance from Shenzhen Longgang Central Hospital. We also express our sincere gratitude to all the parents who volunteered to participate in the study.

Conflict of interest statement

The authors declare they have no competing financial interests.

Author information

Authors and Affiliations

  1. Department of Genetic Toxicology, Shenzhen Center for Disease Control and Prevention, 518020, Shenzhen, People’s Republic of China

    Yuebin Ke, Xiaobei Duan, Xinyun Xu, Gonghua Tao, Li Zhou & Renli Zhang

  2. Department of Pediatrics, Second Clinical College of Medicine, Jinan University, 518020, Shenzhen, People’s Republic of China

    Feiqiu Wen & Baoming Qiu

Authors
  1. Yuebin Ke
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Xiaobei Duan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Feiqiu Wen
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Xinyun Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Gonghua Tao
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Li Zhou
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Renli Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Baoming Qiu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Yuebin Ke.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ke, Y., Duan, X., Wen, F. et al. Association of melamine exposure with urinary stone and oxidative DNA damage in infants. Arch Toxicol 84, 301–307 (2010). https://doi.org/10.1007/s00204-009-0500-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00204-009-0500-5

Keywords

Search

Navigation