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Alishewanella solinquinati sp. nov., Isolated from Soil Contaminated with Textile Dyes

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Abstract

A novel Gram-negative, motile, rod-shaped, facultative anaerobic bacterial strain, KMK6T, was isolated from soil contaminated with textile dyes from an industrial estate located at Ichalkaranji, Maharashtra, India, and its taxonomical position was established by using a polyphasic approach. The major cellular fatty acids included C17:1ω8c, summed feature 3 (C16:1ω7c and/or iso-C15:0 2-OH), C17:0, C16:0, and C18:1ω7c. The DNA G+C content of strain KMK6T was 48.8 mol %. 16S rRNA gene sequence analysis confirmed its placement in the genus Alishewanella, and exhibited sequence similarity levels of below 97 % to the type strains of validly published Alishewanella species. On the basis of genotypic and phenotypic evidence, strains KMK6T is considered to be a novel species of the genus Alishewanella, for which we propose that strain KMK6T (=NCIM 5295T =BCRC 17848T) is assigned to a novel species, Alishewanella solinquinati sp. nov.

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Acknowledgments

The author Y. M. K. thanks to the University Grants Commission (UGC), New Delhi for the research fellowship. We thank Dr. Elke Lang, DSMZ, Germany for her support in the course of the work and for providing the Alishewanella aestuarii DSMZ 19476T required for our study.

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Authors and Affiliations

  1. Biochemistry Division, Department of Chemistry, University of Pune, Ganeshkhind, Pune, 411007, India

    Yogesh M. Kolekar & Kisan M. Kodam

  2. Microbial Culture Collection, National Centre for Cell Science, Pune, 411007, Maharashtra, India

    Shrikant P. Pawar & Yogesh S. Shouche

  3. School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore, 637551, Singapore

    Sunil S. Adav

  4. The Key Laboratory for Microbial Resources of the Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming, 650091, Yunnan, People’s Republic of China

    Liu-Qiang Zheng, Wen-Jun Li & Syed G. Dastager

  5. National Collection of Industrial Microorganisms, Division of Biochemical Sciences, CSIR-National Chemical Laboratory, Pune, 411008, India

    Yogesh M. Kolekar

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  1. Yogesh M. Kolekar
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  2. Shrikant P. Pawar
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Correspondence to Syed G. Dastager.

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Kolekar, Y.M., Pawar, S.P., Adav, S.S. et al. Alishewanella solinquinati sp. nov., Isolated from Soil Contaminated with Textile Dyes. Curr Microbiol 67, 454–459 (2013). https://doi.org/10.1007/s00284-013-0385-7

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  • DOI: https://doi.org/10.1007/s00284-013-0385-7

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