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An electrochemiluminescence aptasensor switch for aldicarb recognition via ruthenium complex-modified dendrimers on multiwalled carbon nanotubes

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Abstract

The authors describe an electrochemiluminescence (ECL) based aptasensor for the pesticide aldicarb. The method is based on effective ECL energy transfer that occurs between the ruthenium(II) bipyridyl complex [referred to as Ru(bpy)3 2+] and gold nanoparticles (AuNPs). More specifically, multiwalled carbon nanotubes were modified with dendritic poly(L-arginine) labeled with Ru(bpy)3 2+, and the aptamers were taggedd with AuNPs. In the absence of aldicarb, the ECL emitted by Ru(bpy)3 2+ is enhanced by AuNPs under peak wavelength at at a wavelength of 610 nm. In the presence of aldicarb, the capture and competitive binding of aldicarb to the DNA aptamers causes their separation from the DPA6/Ru(bpy)3 2+/MWCNT. As a result, ECL intensity decreases linearly with increasing aldicarb concentrations in the range between 40 pM and 4 nM, with a detection limit of 9.6 pM. This aptamer switch is highly sensitive, selective and inexpensive. Conceivably, it can be adapted to formats for the determination of other pesticide residues by using different DNA aptamers.

Schematic of the procedure for aptamer-based detection of aldicarb using the ECL signal of the Ru(bpy)3 2+ amplified by gold nanoparticles. This assay has high sensitivity, good selectivity, and low cost. It can presumably be transferred to other pesticide detection schemes.

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Acknowledgements

The authors gratefully acknowledge the financial support received from the Fundamental Scientific Research Funds for Chinese Academy of Tropical Agricultural Sciences (No. 1630042015011 and 1630042015009) and the Science and Technology Project of Hainan Province (No. ZDXM2015044).

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Authors and Affiliations

  1. Analysis and Test Center of Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, China

    Shuhuai Li, Chunhua Liu, Bingjun Han, Jinhui Luo & Guihao Yin

  2. Hainan Provincial Key Laboratory of Quality and Safety for Tropical Fruits and Vegetables, Haikou, 571101, China

    Shuhuai Li, Chunhua Liu, Bingjun Han, Jinhui Luo & Guihao Yin

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  1. Shuhuai Li
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  2. Chunhua Liu
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  3. Bingjun Han
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  4. Jinhui Luo
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  5. Guihao Yin
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Correspondence to Shuhuai Li or Guihao Yin.

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Li, S., Liu, C., Han, B. et al. An electrochemiluminescence aptasensor switch for aldicarb recognition via ruthenium complex-modified dendrimers on multiwalled carbon nanotubes. Microchim Acta 184, 1669–1675 (2017). https://doi.org/10.1007/s00604-017-2177-4

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  • DOI: https://doi.org/10.1007/s00604-017-2177-4

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