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Amperometric myeloperoxidase immunoassay based on the use of CuPdPt nanowire networks

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Abstract

This research describes a nanowire network-based method for detecting the activity of myeloperoxidase (MPO), a biomarker of acute coronary syndromes (ACS). Trimetallic CuPdPt nanowire networks (CuPdPt NWNWs) were prepared by a one-step chemical reduction method. The metallic precursors quickly form nanowire network structures without the need for additional capping agents or surfactants. This process creates a product with a clean surface. The NWNWs were dropped onto a glassy carbon electrode (GCE) to obtain a sensor with good catalytic activity towards the reduction of hydrogen peroxide (H2O2), which was used as an electrochemical probe working at −0.4 V (vs. SCE). It also provided a large surface for further modification. Next, an antibody against MPO was immobilized on the modified GCE via the stable conjunction between Cu, Pt, Pd and amino groups. Upon binding of MPO to the antibody on the GCE, the current response to H2O2 was reduced by 35 μA·cm−2. The immunosensor had a linear response within the 100 fg·mL−1 to 50 ng·mL−1 MPO concentration range and a 33 fg·mL−1 detection limit (at an S/N ratio of 3). The recovery of spiked serum samples ranged from 99.8 to 103.6%. This result suggests that the method can be applied to the quantitation of MPO in human serum samples.

A trimetallic CuPdPt nanowire networks was placed on a glassy cabon electrode (GCE) to design an immunosensor for myeloperoxidase (MPO), a biomarker for the acute coronary syndrome (ACS). Antibody against MPO was immobilized on the network via conjugation between Cu, Pt, Pd and amino groups. Amperometric i-t measurements were conducted to quantify the amount of MPO that binds to the antibody on the surface of the modified GCE.

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Acknowledgments

We are grateful for the financial support from the National Nature Science Foundation of China (No. 81370403), the Chongqing Foundation and Advanced Research Project (No. CSTC2015jcyjBX0053), the Chongqing Precision Medical Key Technology Research and Development and Demonstration Projects (cstc2016shms-ztzx0042) and the Chongqing Medical University Scientific Research Cultivating Fund (No.201414).

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  1. Jianyong Yuan and Yilin Wen contributed equally to this work.

Authors and Affiliations

  1. College of Pharmacy, and Chongqing Pharmacodynamic Evaluation Engineering Technology Research Center, Chongqing Medical University, Box 380#, 1 Yi Xue Yuan Road, Chongqing, 400016, People’s Republic of China

    Yilin Wen, Jianyong Yuan, Jun Chen, Yilin Zhao, Yazhen Niu & Chao Yu

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  1. Yilin Wen
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  2. Jianyong Yuan
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Correspondence to Chao Yu.

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Wen, Y., Yuan, J., Chen, J. et al. Amperometric myeloperoxidase immunoassay based on the use of CuPdPt nanowire networks. Microchim Acta 185, 55 (2018). https://doi.org/10.1007/s00604-017-2563-y

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