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A graphene modified carbon ionic liquid electrode for voltammetric analysis of the sequence of the Staphylococcus aureus nuc gene

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Abstract

The authors describe a voltammetric method for the detection of the nuc ssDNA sequence originating from Staphylococcus aureus by using a carbon ionic liquid electrode modified with electrodeposited three-dimensional graphene (3DGR). Probe ssDNA was electrostatically adsorbed on the modified electrode by a potentiostatic method. The porous structure and large surface area of 3DGR greatly increase the amount of immobilized probe ssDNA on the interface, which is beneficial for the reaction with target ssDNA. By using Methylene Blue (MB) as the electrochemical probe, the reduction peak current of MB (best measured at −0.30 V vs. SCE) can be used for detecting hybridization. The differential pulse voltammetric current of MB increases linearly in the 1.0 × 10−12 mol L−1 to 1.0 × 10−6 mol L−1 nuc concentration range, and the detection limit is 3.3 × 10−13 mol L−1 (at 3σ). The DNA sensor was successfully applied to the determination of the PCR product of the gene in pork.

Response of an electrochemical DNA biosensor based on the use of a carbon ionic liquid electrode modified with three-dimensional graphene. It enables sensitive voltammetric detection of the specific sequence of the Staphylococcus aureus nuc gene.

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Acknowledgements

The financial support by the National Natural Science Foundation of China (21665007), the Program for Innovative Research Team in University (IRT-16R19), the Natural Science Foundation of Hainan Province (2017CXTD007), the Key Science and Technology Program of Haikou City (2017042) and the Fundamental Research Funds for the Central Universities (30916014103) are gratefully acknowledged.

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Authors and Affiliations

  1. Key Laboratory of Tropical Medicinal Plant Chemistry of Ministry of Education, College of Chemistry and Chemical Engineering, Hainan Normal University, Haikou, 571158, People’s Republic of China

    Xueliang Niu, Wei Chen, Yongling Men, Qin Wang & Wei Sun

  2. College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, People’s Republic of China

    Wei Chen, Xiuli Wang & Guangjiu Li

  3. Key Laboratory of Soft Chemistry and Functional Materials of Ministry of Education, College of Chemical Engineering, Nanjing University of Science and Technology, Nanjing, 210094, People’s Republic of China

    Wei Sun

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  1. Xueliang Niu
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Correspondence to Wei Sun.

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Niu, X., Chen, W., Wang, X. et al. A graphene modified carbon ionic liquid electrode for voltammetric analysis of the sequence of the Staphylococcus aureus nuc gene. Microchim Acta 185, 167 (2018). https://doi.org/10.1007/s00604-018-2719-4

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