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Analysis of the complete genome sequence of a potyvirus from passion fruit suggests its taxonomic classification as a member of a new species

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A Correction to this article was published on 17 November 2020

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Abstract

The complete genomic sequence of a telosma mosaic virus (TeMV) isolate (named PasFru), identified in passion fruit in China, was determined. The entire RNA genome of PasFru comprises 10,049 nucleotides (nt) excluding the poly(A) tail and encodes a polyprotein of 3,173 amino acids (aa), flanked by 5’ and 3’ untranslated regions (UTR) of 276 and 251 nt, respectively. Compared with the previous TeMV isolate Hanoi from Telosma cordata, the only documented isolate with the entire genome sequence annotated, PasFru had an extra 87 nt and 89 aa residues at the 3’-end of 5’UTR and the N-terminus of the P1 protein, respectively, which contributed to the genome size difference between PasFru and Hanoi (10,049 nt versus 9,689 nt). Pairwise sequence comparisons showed that PasFru shares 73.6% nt and 80.9% aa sequence identity with the Hanoi isolate at the whole-genome and polyprotein level, respectively, and these values are below the corresponding threshold values for species demarcation in the family Potyviridae. These data suggest that TeMV-PasFru should be classified as a new member of the genus Potyvirus.

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Change history

  • 17 November 2020

    Recently, we carefully checked the original Sanger sequencing data for the assembly

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Acknowledgements

The authors thank Dr. Xiaofei Cheng (Northeast Agricultural University) for suggestions in analysis of sRNA data, and Ye Li (Chinese Academy of Tropical Agricultural Sciences) for assistance with transmission electron micrography. This study was supported financially by Hainan Provincial Natural Science Foundation of China (Grant No. 318MS006), Central Public-Interest Scientific Institution Basal Research Fund (Grant No. 2017hzs1J022), and the Scientific Research Foundation for Advanced Talents, Hainan University (Grant No. KYQD(ZR)1734).

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Authors and Affiliations

  1. College of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan, 570228, People’s Republic of China

    Ke Yang, Pengfei Jin, Weiguo Miao & Hongguang Cui

  2. College of Forestry, Agricultural University of Hebei, Baoding, Hebei, 071001, People’s Republic of China

    Haixia Yan

  3. College of International Cultural Exchange, Hainan University, Haikou, Hainan, 570228, People’s Republic of China

    Li Song

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  1. Ke Yang
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  2. Haixia Yan
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  3. Li Song
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  4. Pengfei Jin
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  5. Weiguo Miao
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  6. Hongguang Cui
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Correspondence to Hongguang Cui.

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Handling Editor: F. Murilo Zerbini.

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Yang, K., Yan, H., Song, L. et al. Analysis of the complete genome sequence of a potyvirus from passion fruit suggests its taxonomic classification as a member of a new species. Arch Virol 163, 2583–2586 (2018). https://doi.org/10.1007/s00705-018-3885-8

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  • DOI: https://doi.org/10.1007/s00705-018-3885-8

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