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A hydrogen peroxide biosensor based on the direct electron transfer of hemoglobin in the nanosheets of exfoliated HNb3O8

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Abstract

In the present work, hemoglobin (Hb) was entrapped into the nanosheets of a pre-exfoliated layered material HNb3O8. UV–vis spectra analysis displayed that no significant denaturation occurred to the entrapped protein. Electrochemical results showed that the entrapment of Hb into layered HNb3O8 enhanced the direct electron transfer ability between protein molecules and electrode. A pair of well-defined redox peaks was observed at −0.39 and −0.34 V on the glassy carbon electrode modified with the Hb/HNb3O8 composite. The electrode reactions showed a surface-controlled process with a single electron transfer at the scan rate of 50–400 mV/s, and the electron transfer rate was very fast. The entrapped Hb retained its biological activity well and the sensor constructed by the Hb/HNb3O8-composite-modified electrode displayed excellent response to the reduction of hydrogen peroxide (H2O2) with wide linear range, low detection limit, and good stability.

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Acknowledgments

The work is supported by the Fund on Basic Scientific Research Project of Nonprofit Central Research Institutions (no. SSCRI200901), and the Natural science Foundation of Hainan province (no. 310099).

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Authors and Affiliations

  1. South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Science, Zhanjiang, 524091, China

    Yu-Ge Liu, Chang-bin Wei, Ling-Ling Lv & Sheng-Hui Liu

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  1. Yu-Ge Liu
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  2. Chang-bin Wei
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  3. Ling-Ling Lv
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  4. Sheng-Hui Liu
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Correspondence to Yu-Ge Liu.

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Liu, YG., Wei, Cb., Lv, LL. et al. A hydrogen peroxide biosensor based on the direct electron transfer of hemoglobin in the nanosheets of exfoliated HNb3O8 . J Solid State Electrochem 16, 2211–2216 (2012). https://doi.org/10.1007/s10008-012-1649-z

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  • DOI: https://doi.org/10.1007/s10008-012-1649-z

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