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Activation of salicylic acid metabolism and signal transduction can enhance resistance to Fusarium wilt in banana (Musa acuminata L. AAA group, cv. Cavendish)

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Abstract

Fusarium wilt caused by the fungus Fusarium oxysporum f. sp. cubens (Foc) is the most serious disease that attacks banana plants. Salicylic acid (SA) can play a key role in plant–microbe interactions. Our study is the first to examine the role of SA in conferring resistance to Foc TR4 in banana (Musa acuminata L. AAA group, cv. Cavendish), which is the greatest commercial importance cultivar in Musa. We used quantitative real-time reverse polymerase chain reaction (qRT-PCR) to analyze the expression profiles of 45 genes related to SA biosynthesis and downstream signaling pathways in a susceptible banana cultivar (cv. Cavendish) and a resistant banana cultivar (cv. Nongke No. 1) inoculated with Foc TR4. The expression of genes involved in SA biosynthesis and downstream signaling pathways was suppressed in a susceptible cultivar and activated in a resistant cultivar. The SA levels in each treatment arm were measured using high-performance liquid chromatography. SA levels were decreased in the susceptible cultivar and increased in the resistant cultivar. Finally, we examined the contribution of exogenous SA to Foc TR4 resistance in susceptible banana plants. The expression of genes involved in SA biosynthesis and signal transduction pathways as well as SA levels were significantly increased. The results suggest that one reason for banana susceptibility to Foc TR4 is that expression of genes involved in SA biosynthesis and SA levels are suppressed and that the induced resistance observed in banana against Foc TR4 might be a case of salicylic acid-dependent systemic acquired resistance.

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Acknowledgments

This work was supported by earmarked funds for the Modern Agro-industry Technology Research System of China (CARS-32), the National Natural Science Foundation of China (NNSFC) (31071788), National Nonprofit Institute Research Grant of CATAS (1630052014009), and the Major Technology Project of Hainan (ZDZX2013023-1).

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Authors and Affiliations

  1. Key Laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan, 571101, China

    Zhuo Wang, Caihong Jia, Suzhen Huang, Biyu Xu & Zhiqiang Jin

  2. Haikou Experimental Station, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan, 570101, China

    Jingyang Li & Zhiqiang Jin

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  1. Zhuo Wang
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  2. Caihong Jia
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  3. Jingyang Li
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  4. Suzhen Huang
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  5. Biyu Xu
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  6. Zhiqiang Jin
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Correspondence to Biyu Xu or Zhiqiang Jin.

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Zhuo Wang and Caihong Jia contributed equally to this work.

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Wang, Z., Jia, C., Li, J. et al. Activation of salicylic acid metabolism and signal transduction can enhance resistance to Fusarium wilt in banana (Musa acuminata L. AAA group, cv. Cavendish). Funct Integr Genomics 15, 47–62 (2015). https://doi.org/10.1007/s10142-014-0402-3

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