Skip to main content
SpringerLink
Log in

Angiotensinogen T174M and M235T Variants and Hypertension in the Hani and Yi Minority Groups of China

  • Published:
Biochemical Genetics Aims and scope Submit manuscript

Abstract

A case–control study of 538 individuals investigated whether the angiotensinogen gene (AGT) might be implicated in the pathogenesis of essential hypertension in the Hani and Yi populations of China. Genotypes for two diallelic DNA polymorphisms observed at amino acid residues 174 (T174M) and 235 (M235T) within the coding sequence were determined. M235T and T174M genotyping with PCR-RFLP was performed in 267 normotensive subjects and 271 hypertensive subjects. No significant difference was found between normotensives and hypertensives in genotype distribution and allele frequency for either M235T or T174M in the Hani or the Yi populations (P > 0.05). Relative to carriers of the 235T/235T and 174T/174T combination, the others had a significantly elevated risk of hypertension (OR = 1.62, 95% CI 1.02–2.59; P = 0.043) in the Hani population. The AGT M235T and T174M variants in combination may play a role in the genetic predisposition to develop essential hypertension in the Hani minority of China.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Barley J, Blackwood A, Sagnella G, Markandu N, MacGregor G, Carter N (1994) Angiotensinogen Met235>Thr polymorphism in a London normotensive and hypertensive black and white population. J Hum Hypertens 8(8):639–640

    CAS  PubMed  Google Scholar 

  • Borecki IB, Province MA, Ludwig EH, Ellison RC, Folsom AR, Heiss G, Lalouel JM, Higgins M, Rao DC (1997) Associations of candidate loci angiotensinogen and angiotensin-converting enzyme with severe hypertension: the NHLBI family heart study. Ann Epidemiol 7(1):13–21

    Article  CAS  PubMed  Google Scholar 

  • Caulfield M, Lavender P, Farrall M, Munroe P, Lawson M, Turner P, Clark AJ (1994) Linkage of the angiotensinogen gene to essential hypertension. N Engl J Med 330(23):1629–1633

    Article  CAS  PubMed  Google Scholar 

  • Caulfield M, Lavender P, Newell-Price J, Farrall M, Kamdar S, Daniel H, Lawson M, De Freitas P, Fogarty P, Clark AJ (1995) Linkage of the angiotensinogen gene locus to human essential hypertension in African Caribbeans. J Clin Invest 96(2):687–692

    Article  CAS  PubMed  Google Scholar 

  • Fornage M, Turner ST, Sing CF, Boerwinkle E (1995) Variation at the M235T locus of the angiotensinogen gene and essential hypertension: a population-based case-control study from Rochester, Minnesota. Hum Genet 96(3):295–300

    Article  CAS  PubMed  Google Scholar 

  • Gu CC, Chang YP, Hunt SC, Schwander K, Arnett D, Djousse L, Heiss G, Oberman A, Lalouel JM, Province M, Chakravarti A, Rao DC (2005) Haplotype association analysis of AGT variants with hypertension-related traits: the HyperGEN study. Hum Hered 60(3):164–176

    Article  CAS  PubMed  Google Scholar 

  • Hata A, Namikawa C, Sasaki M, Sato K, Nakamura T, Tamura K, Lalouel JM (1994) Angiotensinogen as a risk factor for essential hypertension in Japan. J Clin Invest 93(3):1285–1287

    Article  CAS  PubMed  Google Scholar 

  • Havlik RJ, Garrison RJ, Feinleib M, Kannel WB, Castelli WP, McNamara PM (1979) Blood pressure aggregation in families. Am J Epidemiol 110(3):304–312

    CAS  PubMed  Google Scholar 

  • Hegele RA, Brunt JH, Connelly PW (1994) A polymorphism of the angiotensinogen gene associated with variation in blood pressure in a genetic isolate. Circulation 90(5):2207–2212

    CAS  PubMed  Google Scholar 

  • Hegele RA, Brunt JH, Connelly PW (1996) Genetic and biochemical factors associated with variation in blood pressure in a genetic isolate. Hypertension 27(2):308–312

    CAS  PubMed  Google Scholar 

  • Hingorani AD, Sharma P, Jia H, Hopper R, Brown MJ (1996) Blood pressure and the M235T polymorphism of the angiotensinogen gene. Hypertension 28(5):907–911

    CAS  PubMed  Google Scholar 

  • Iwai N, Shimoike H, Ohmichi N, Kinoshita M (1995) Angiotensinogen gene and blood pressure in the Japanese population. Hypertension 25(4 pt 2):688–693

    CAS  PubMed  Google Scholar 

  • Jeunemaitre X, Soubrier F, Kotelevtsev YV, Lifton RP, Williams CS, Charru A, Hunt SC, Hopkins PN, Williams RR, Lalouel JM et al (1992) Molecular basis of human hypertension: role of angiotensinogen. Cell 71(1):169–180

    Article  CAS  PubMed  Google Scholar 

  • Jeunemaitre X, Inoue I, Williams C, Charru A, Tichet J, Powers M, Sharma AM, Gimenez-Roqueplo AP, Hata A, Corvol P, Lalouel JM (1997) Haplotypes of angiotensinogen in essential hypertension. Am J Hum Genet 60(6):1448–1460

    Article  CAS  PubMed  Google Scholar 

  • Johnson AG, Simons LA, Friedlander Y, Simons J, Davis DR, MaCallum J (1996) M235>T polymorphism of the angiotensinogen gene predicts hypertension in the elderly. J Hypertens 14(9):1061–1065

    Article  CAS  PubMed  Google Scholar 

  • Kamitani A, Rakugi H, Higaki J, Yi Z, Mikami H, Miki T, Ogihara T (1994) Association analysis of a polymorphism of the angiotensinogen gene with essential hypertension in Japanese. J Hum Hypertens 8(7):521–524

    CAS  PubMed  Google Scholar 

  • Levine RS, Hennekens CH, Perry A, Cassady J, Gelband H, Jesse MJ (1982) Genetic variance of blood pressure levels in infant twins. Am J Epidemiol 116(5):759–764

    CAS  PubMed  Google Scholar 

  • Lifton RP, Gharavi AG, Geller DS (2001) Molecular mechanisms of human hypertension. Cell 104(4):545–556

    Article  CAS  PubMed  Google Scholar 

  • Martin ER, Lai EH, Gilbert JR, Rogala AR, Afshari AJ, Riley J, Finch KL, Stevens JF, Livak KJ, Slotterbeck BD, Slifer SH, Warren LL, Conneally PM, Schmechel DE, Purvis I, Pericak-Vance MA, Roses AD, Vance JM (2000) SNPing away at complex diseases: analysis of single-nucleotide polymorphisms around APOE in Alzheimer disease. Am J Hum Genet 67(2):383–394

    Article  CAS  PubMed  Google Scholar 

  • Martinez E, Puras A, Escribano J, Sanchis C, Carrion L, Artigao M, Divison JA, Masso J, Fernandez JA (2002) Threonines at position 174 and 235 of the angiotensinogen polypeptide chain are related to familial history of hypertension in a Spanish-Mediterranean population. Br J Biomed Sci 59(2):95–100

    CAS  PubMed  Google Scholar 

  • Nakamura Y, Tabara Y, Miki T, Tamaki S, Kita Y, Okamura T, Ueshima H (2007) Both angiotensinogen M235T and alpha-adducin G460 W polymorphisms are associated with hypertension in the Japanese population. J Hum Hypertens 21(3):253–255

    CAS  PubMed  Google Scholar 

  • Nishiuma S, Kario K, Kayaba K, Nagio N, Shimada K, Matsuo T, Matsuo M (1995) Effect of the angiotensinogen gene Met235>Thr variant on blood pressure and other cardiovascular risk factors in two Japanese populations. J Hypertens 13(7):717–722

    Article  CAS  PubMed  Google Scholar 

  • Niu T, Chen C, Yang J, Wang B, Wang Z, Schork N, Fang Z, Xu X (1999) Blood pressure and the T174 M and M235T polymorphisms of the angiotensinogen gene. Ann Epidemiol 9(4):245–253

    Article  CAS  PubMed  Google Scholar 

  • Qi Y, Niu W, Zhou W, Hou S, Qiu C (2007) Correlation between angiotensinogen gene polymorphisms and essential hypertension in Chinese population. J Hum Hypertens 22:147–150

    Article  PubMed  Google Scholar 

  • Renner W, Nauck M, Winkelmann BR, Hoffmann MM, Scharnagl H, Mayer V, Boehm BO, Marz W (2005) Association of angiotensinogen haplotypes with angiotensinogen levels but not with blood pressure or coronary artery disease: the Ludwigshafen Risk and Cardiovascular Health Study. J Mol Med 83(3):235–239

    Article  CAS  PubMed  Google Scholar 

  • Rotimi C, Morrison L, Cooper R, Oyejide C, Effiong E, Ladipo M, Osotemihen B, Ward R (1994) Angiotensinogen gene in human hypertension: lack of an association of the 235T allele among African Americans. Hypertension 24(5):591–594

    CAS  PubMed  Google Scholar 

  • Schmidt S, Sharma AM, Zilch O, Beige J, Walla-Friedel M, Ganten D, Distler A, Ritz E (1995) Association of M235T variant of the angiotensinogen gene with familial hypertension of early onset. Nephrol Dial Transplant 10(7):1145–1148

    CAS  PubMed  Google Scholar 

  • Sethi AA, Nordestgaard BG, Agerholm-Larsen B, Frandsen E, Jensen G, Tybjaerg-Hansen A (2001) Angiotensinogen polymorphisms and elevated blood pressure in the general population: the Copenhagen City Heart Study. Hypertension 37(3):875–881

    CAS  PubMed  Google Scholar 

  • Sethi AA, Nordestgaard BG, Tybjaerg-Hansen A (2003) Angiotensinogen gene polymorphism, plasma angiotensinogen, and risk of hypertension and ischemic heart disease: a meta-analysis. Arterioscler Thromb Vasc Biol 23(7):1269–1275

    Article  CAS  PubMed  Google Scholar 

  • Tang W, Wu H, Zhou X, Cheng B, Dong Y, He L, Yu H, Xu L, Lu J, Li K, Xiao C (2006) Association of the C-344T polymorphism of CYP11B2 gene with essential hypertension in Hani and Yi minorities of China. Clin Chim Acta 364(1–2):222–225

    Article  CAS  PubMed  Google Scholar 

  • Vasku A, Soucek M, Znojil V, Rihacek I, Tschoplova S, Strelcova L, Cidl K, Blazkova M, Hajek D, Holla L, Vacha J (1998) Angiotensin I-converting enzyme and angiotensinogen gene interaction and prediction of essential hypertension. Kidney Int 53(6):1479–1482

    Article  CAS  PubMed  Google Scholar 

  • Wang WY, Glenn CL, Zhang W, Benjafield AV, Nyholt DR, Morris BJ (1999) Exclusion of angiotensinogen gene in molecular basis of human hypertension: sibpair linkage and association analyses in Australian anglo-caucasians. Am J Med Genet 87(1):53–60

    Article  CAS  PubMed  Google Scholar 

  • Wu H, Tang W, Li H, Zhou X, Yang Y, Yu H, Li K, Xiao C, Deng AY (2006) Association of the beta2-adrenergic receptor gene with essential hypertension in the non-Han Chinese Yi minority human population. J Hypertens 24(6):1041–1047

    Article  CAS  PubMed  Google Scholar 

  • Zhao H, Pfeiffer R, Gail MH (2003) Haplotype analysis in population genetics and association studies. Pharmacogenomics 4(2):171–178

    Article  PubMed  Google Scholar 

Download references

Acknowledgments

This work was financially supported by the National Natural Science Foundation of China (No. 30660076) and the 973 Prophase Project of the Science and Technology Ministry of China and the Science and Technology Bureau of Yunnan Province (No. 2006CB708502 and No. 2006GP10).

Author information

Authors and Affiliations

  1. Laboratory for Conservation and Utilization of Bio-resources, Human Genetics Center of Yunnan University, 2 N. Cuihu Rd, Kunming, Yunnan, 650091, China

    Wenru Tang, Yinan Chun, Yang Yang & Chunjie Xiao

  2. Basic Medical School of Yunnan College of Traditional Chinese Medicine, 201 Shuangqiao Rd., Guanshang, Kunming, Yunnan, 650200, China

    Jiali Yuan & Hua Ying

Authors
  1. Jiali Yuan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Wenru Tang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yinan Chun
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Hua Ying
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yang Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Chunjie Xiao
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Chunjie Xiao.

Additional information

Jiali Yuan and Wenru Tang contributed equally to this work.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yuan, J., Tang, W., Chun, Y. et al. Angiotensinogen T174M and M235T Variants and Hypertension in the Hani and Yi Minority Groups of China. Biochem Genet 47, 344–350 (2009). https://doi.org/10.1007/s10528-009-9237-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10528-009-9237-3

Keywords

Search

Navigation