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A Simple and Rapid Resonance Scattering Spectral Method for Detection of Trace Hg2+ Using Aptamer-Nanogold as Probe

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Abstract

Single-strand deoxyribonucleic acid (ssDNA) were used to modified nanogold particle to obtain a aptamer-nanogold probe (NGssDNA) for Hg(II). The probe is not aggregated in high concentration of NaCl. In the pH 7.0 Na2HPO4-NaH2PO4 buffer solution and in the presence of high concentration of NaCl, NGssDNA interact with Hg(II) to form stable double-strand T-Hg(II)-T mismatches and to release nanogold particles from the probe. The released nanogold particles aggregated to form bigger clusters which leaded the resonance scattering (RS) intensity at 540 nm enhanced linearly with the concentration of Hg2+ in the range of 0.39–1666.7 nM, with detection of 0.1 nM. This simple, rapid, and sensitive aptamer-nanogold RS assay was applied to determination of Hg2+ in wastewater, with satisfactory results.

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Authors and Affiliations

  1. Key Laboratory of Ecology of Rare and Endangered Species, School of Environment and Resource, Guangxi Normal University, Guilin, 541004, Guangxi Province, China

    Guiqing Wen, Aihui Liang, Yanyan Fan & Zhiliang Jiang

  2. Environmental Conservation of Education Ministry, School of Environment and Resource, Guilin University of Technology, Guilin, 541004, Guangxi Province, China

    Guiqing Wen, Aihui Liang, Yanyan Fan & Zhiliang Jiang

  3. Department of Applied Chemistry, Guilin University of Technology, Guilin, 541004, China

    Caina Jiang

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  1. Guiqing Wen
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  2. Aihui Liang
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  3. Yanyan Fan
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  4. Zhiliang Jiang
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  5. Caina Jiang
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Correspondence to Zhiliang Jiang.

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Wen, G., Liang, A., Fan, Y. et al. A Simple and Rapid Resonance Scattering Spectral Method for Detection of Trace Hg2+ Using Aptamer-Nanogold as Probe. Plasmonics 5, 1–6 (2010). https://doi.org/10.1007/s11468-009-9107-1

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  • DOI: https://doi.org/10.1007/s11468-009-9107-1

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