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Anti-inflammatory effect of Qingwen Baidu Decoction (清瘟败毒饮) in sepsis rats

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Abstract

Objective

To explore the pharmacological anti-inflammatory mechanism of Chinese formula Qingwen Baidu Decoction (清瘟败毒饮, QBD) from the view of holistic biology.

Methods

The rats were randomly divided into a normal conrol group, a lipopolysaccharide (LPS) group, the low- and high-dose QBD groups, and a dexamethasone (DXM) group. NR8383 cells were treated with culture fluid containing 6% serum from rats of each group respectively. Inflammatory mediators were detected by reverse transcription polymerase chain reaction (RT-PCR), Western blotting hybridization, enzyme linked immunosorbent assay (ELISA), polymerase chain reaction (PCR) gene array and antibody array.

Results

It is showed that the levels of interleukin (IL)-1α, IL-4 and IL-12 were enhanced in the low-dose QBD group; levels of IL-1α, IL-12 and IL-18 were augmented in the high-dose QBD group, compared with the LPS group after ELISA detection. Western blot showed that IL-1β and tumor necrosis factor (TNF)-α expression of the control group were lower than other groups. IL-1β level of the low-dose and high-dose QBD groups detected by RT-PCR was higher in early stage but lower after 24 h than that of the control group (P<0.01). Expression of 84 main inflammatory cytokines and receptors was detected by rat inflammatory cytokines and receptors PCR array. Up-regulation genes were 22 in both the LPS group and the low-dose QBD group, among which 16 up-regulating genes were the same. In these 16 genes, the up-regulating amplitude of 9 genes in the low-dose QBD group was less than that in the LPS group, 4 were similar to and 3 were more. Twenty-nine main cytokines were inspected by rat cytokine antibody array. Intergroup gray value differences were found in 7 expressed cytokines. The levels of these 7 cytokines in the lowdose QBD group were all lower than those in the the LPS group.

Conclusions

QBD has anti-inflammatory effect on sepsis by changing the level of inflammatory mediators.

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Authors and Affiliations

  1. Department of Cerebropathia, the First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510405, China

    Zheng-miao Yu  (于征淼)

  2. Department of Clinical Laboratory, the First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, 510405, China

    Zhi-hui Liu  (刘志辉)

  3. Hospital of Guangdong University of Technology, Guangzhou, 510090, China

    Jing Chen  (陈 晶)

  4. Library of Guangzhou University of Chinese Medicine, Guangzhou, 510405, China

    Qiang Zeng  (曾 强)

Authors
  1. Zheng-miao Yu  (于征淼)
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  2. Zhi-hui Liu  (刘志辉)
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  3. Jing Chen  (陈 晶)
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  4. Qiang Zeng  (曾 强)
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Corresponding author

Correspondence to Zheng-miao Yu  (于征淼).

Additional information

Supported by National Natural Science Foundation of China (No. 30801468)

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Yu, Zm., Liu, Zh., Chen, J. et al. Anti-inflammatory effect of Qingwen Baidu Decoction (清瘟败毒饮) in sepsis rats. Chin. J. Integr. Med. 20, 934–943 (2014). https://doi.org/10.1007/s11655-014-1863-x

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  • DOI: https://doi.org/10.1007/s11655-014-1863-x

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