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A role for anti-CD45RB monoclonal antibody treatment upon dendritic cells

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Abstract

Selective interference with CD45RB isoform by monoclonal antibody (anti-CD45RBmAb) reliably induces donor-specific tolerance. Dendritic cells (DCs) are the most potent antigen-presenting cells that are capable of activating naïve T cells. The purposes of the present study were to investigate the roles of anti-CD45RBmAb on the phenotypes and functioning of DCs and to further illustrate the mechanism of anti-CD45RBmAb-inducing immunologic tolerance. DCs from C57BL/6 mice were cultured and treated with various doses of anti-CD45RB monoclonal antibody. Cell phenotype, cycle and phagocytic ability were detected by flow cytometry. The production of IL-10 and IL-12 in the supernatants of mature DCs was measured with ELISA. Exosomes (Dex) were recovered from the supernatant of DCs cultured for 6 days in depleted medium, and effects of DCs and Dex on the ability of T-cell proliferation were detected by mixed lymphocyte culture. Anti-CD45RBmAb could inhibit DCs maturation in a dose-dependent manner, and the effects of exosomes (Dex) on DCs enhance or inhibition proliferation of T cells were also in a dose-dependent manner. Anti-CD45RBmAb could profoundly inhibit the maturation and functioning of DCs and generate tolerogenic dendritic cells (tDCs) as well as Dex, suggesting mechanistic contributions to tolerance development from the DCs through interactions with T cells.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 30772042), The Natural Science Foundation of Guangdong (No. 6027540) and The Science and Technology Project of Shenzhen (No. 200601017) and the Medical Science and Technology Foundation of Guangdong (No. B2008158).

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No competing financial interests exist.

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Authors and Affiliations

  1. Clinical Medical Research Center, The Second Clinical Medical College, Shenzhen People’s Hospital, Ji’nan University, No. 1017 Dongmen North Road, Shenzhen, 518020, People’s Republic of China

    Hui Qi, Jin-Peng Liu, Chun-Yan Deng, Han-Xin Zhou, Shao-Ping Deng & Fu-Rong Li

  2. Shenzhen Institute of Gerontology, Shenzhen, 518020, People’s Republic of China

    Fu-Rong Li

  3. Department of Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA

    Shao-Ping Deng

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  1. Hui Qi
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  2. Jin-Peng Liu
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  3. Chun-Yan Deng
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  4. Han-Xin Zhou
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  5. Shao-Ping Deng
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Correspondence to Shao-Ping Deng or Fu-Rong Li.

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Qi, H., Liu, JP., Deng, CY. et al. A role for anti-CD45RB monoclonal antibody treatment upon dendritic cells. Immunol Res 52, 250–257 (2012). https://doi.org/10.1007/s12026-012-8336-0

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