Abstract
A new method for the analysis of α-tocopherol in tropical fruits by ultra performance convergence chromatography (UPC2) was developed for the first time. Five varieties of tropical fruit samples were separately saponified under classical heating and extracted with ether. The extracted α-tocopherol was separated on a BEH column, with a mobile phase consisting of CO2 and methanol, with a gradient elution (99:1 to 90:10), and detected with diode array detector at 293 nm. The limit of detection (LOD) and limit of quantification (LOQ) were about 60.0 and 103.3 ng, respectively. This method was considered to be simple, fast and reliable, and successfully applied to analysis of α-tocopherol in tropical fruits. The values of α-tocopherol in pitaya, jackfruit, durians, mango, and papaya ranged from 0.16 to 0.45 mg/100 g dry weight in edible portion. Recovery rates obtained by the standard addition method on these tropical fruit samples ranged from 95.4 to 101.4 % with high repeatability (RSD, 1.2–2.6 %).
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Acknowledgments
This work was supported by the Special Fund for Agro-scientific Research in the Public Interest (201303077).
Compliance with Ethics Requirements
This is an original research article that has neither been published previously, nor considered presently for publication elsewhere. And all authors named in the manuscript are entitled to the authorship and have approved the final version of the submitted manuscript.
Conflict of Interest
Xiao Gong declares that he has no conflict of interest. Ningli Qi declares that she has no conflict of interest. Xiaoxi Wang declares that she has no conflict of interest. Jihua Li has received research grants (Special Fund for Agro-scientific Research in the Public Interest (201303077)) from the Ministry of Agriculture of the People’s Republic of China. Lijing Lin declares that he has no conflict of interest. This article does not contain any studies with human or animal subjects.
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Gong, X., Qi, N., Wang, X. et al. A New Method for Determination of α-Tocopherol in Tropical Fruits by Ultra Performance Convergence Chromatography with Diode Array Detector. Food Anal. Methods 7, 1572–1576 (2014). https://doi.org/10.1007/s12161-014-9789-7
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DOI: https://doi.org/10.1007/s12161-014-9789-7