Issue 3, 2012
From the journal:

Analyst

A resonance light scattering quenching system for studying DNA sequence recognition of actinomycin D†

Zhanguang Chen,*a   Guomin Zhang,a   Xi Chen,b   Junhui Chen,c   Sihua Qiana  and  Qiang Lia  

* Corresponding authors

a Department of Chemistry, Shantou University, Shantou, China
E-mail: kqlu@stu.edu.cn
Fax: +86 75482902767
Tel: +86 75482903330

b Guangdong Pharmaceutical University, Guangzhou, China

c Department of Radiology, Peking University Shenzhen Hospital, Shenzhen, China

Abstract

The DNA sequence recognition study of DNA-targeted anticancer drugs is a theoretical basis for improving the selectivity of anticancer drugs. With the high synergy effect of cocoamidopropyl hydroxy sulfobetaine (HSB), a resonance light scattering (RLS) quenching system for DNA sequence recognition studies of actinomycin D (ACTD) was developed in this contribution. By the strategy, DNA sequence selectivity as well as the recognition mechanisms of ACTD was systematically investigated. The results suggested that ACTD had the selectivity to single-stranded DNA (ssDNA) with an equilibrium constant (KRLS) of 12.4 mmol mg−1. Also it had a preference for Guanine and Cytosine bases with a KRLS of 6.69 L mmol−1. The selectivity mechanism between ACTD and DNA was also well discussed with the help of UV-Vis absorption spectroscopy. Compared with other methods, the RLS quenching system has the advantages of reliability and speediness, and it avoids complex modification processes and is a better bionic system for the above research. Results obtained from this work would supply a theoretical basis for improving anticancer activity and designing similar anticancer drugs.

Graphical abstract: A resonance light scattering quenching system for studying DNA sequence recognition of actinomycin D

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C1AN15855D
Article type
Paper
Submitted
14 Sep 2011
Accepted
08 Nov 2011
First published
13 Dec 2011

Analyst, 2012,137, 722-728

Permissions

Request permissions

A resonance light scattering quenching system for studying DNA sequence recognition of actinomycin D

Z. Chen, G. Zhang, X. Chen, J. Chen, S. Qian and Q. Li, Analyst, 2012, 137, 722 DOI: 10.1039/C1AN15855D

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements