A fluorescent ratiometric Cu2+ probe based on FRET by naphthalimide-appended rhodamine derivatives

Chunwei Yu; Yingying Wen; Xi Qin; Jun Zhang

doi:10.1039/C4AY01863J

A fluorescent ratiometric Cu²⁺ probe based on FRET by naphthalimide-appended rhodamine derivatives†

Chunwei Yu,^a Yingying Wen,^a Xi Qin*^b and Jun Zhang*^a

* Corresponding authors

^a Laboratory of Environmental Monitoring, School of Tropical and Laboratory Medicine, Hainan Medical University, Haikou, China
E-mail: jun_zh1979@163.com
Fax: +86 898 66989173
Tel: +86 898 66973190

^b Department of Clinical Laboratory, Affiliated Hospital of Hainan Medical University, Haikou 570102, China
E-mail: qinxi99@21cn.com
Fax: +86 898 66989173
Tel: +86 898 66773727

Abstract

A fluorophore dyad (L) containing rhodamine B and naphthalimide units linked through C [double bond, length as m-dash] N was constructed as a ratiometric probe for Cu²⁺. The selective fluorescent response of the probe L to Cu²⁺ was triggered by a Cu²⁺ induced ring-opening process of the rhodamine B spirolactam, which was based on an intramolecular fluorescence resonance energy transfer (FRET) mechanism from naphthalimide to rhodamine B. The system exhibited high selectivity for Cu²⁺ as compared to other tested metal ions and anions, and signaled the binding event through occurrence of FRET mediated emission at 580 nm of the rhodamine chromophore with enhancement in the fluorescence intensity, concurrently, the featured emission of the naphthalimide donor at around 525 nm was nearly completely quenched. Furthermore, it has been used for ratiometric imaging of Cu²⁺ in living cells with satisfying results.

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Article information

DOI: https://doi.org/10.1039/C4AY01863J
Article type: Paper
Submitted: 07 Aug 2014
Accepted: 06 Oct 2014
First published: 06 Oct 2014

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Anal. Methods, 2014,6, 9825-9830

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A fluorescent ratiometric Cu²⁺ probe based on FRET by naphthalimide-appended rhodamine derivatives

C. Yu, Y. Wen, X. Qin and J. Zhang, Anal. Methods, 2014, 6, 9825 DOI: 10.1039/C4AY01863J

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Analytical Methods