Abstract
Tomato is an ideal model species for fleshy fruit development research. SlYABBY2b regulates the ovary locule number, which is increased by gibberellins, in tomato. However, the relationship between SlYABBY2b and endogenous gibberellin is poorly understood. In this study, SlYABBY2b-overexpressing and RNA interference (RNAi) transgenic tomato plants were used to elucidate the mechanism by which SlYABBY2b regulates the ovary locule number and endogenous gibberellin content in tomato. SlYABBY2b-overexpressing plants showed fewer locules and lower gibberellin content than the control plants. Contrasting results were found in the RNAi lines. Therefore, the SlYABBY2b gene negatively regulates tomato ovary locule number and endogenous gibberellin content. Furthermore, the expression of SlYABBY2b gene was remarkably higher than that of the wild type in the apical shoots of gibberellindeficient mutants. This showed that the gibberellins can inhibit the expression of SlYABBY2b gene negative regulation. Further study revealed that SlYABBY2b suppressed the expression of SlGA20ox1 and SlGA3ox2, but increased that of SlGA2ox1 and SlGA2ox5 in the apical shoots of SlYABBY2b-overexpressing plants, thereby reducing gibberellin content. Contrasting results were found in the RNAi lines. Our results showed that the SlYABBY2b gene was located on gibberellin signal transduction pathways, fed back regulation of the synthesis of gibberellin, and felt exogenous gibberellin signal to further regulate the formation of tomato locule.
摘要
目的
探讨赤霉素对SlYABBY2b 基因调控番茄心室形成 过程中的作用,为进一步研究番茄畸形果发生机 理提供了理论基础。
创新点
首次明确了SlYABBY2b 基因与赤霉素的关系,且筛选出SlYABBY2b 调控赤霉素合成的关键基因 GA20ox1、GA3ox2、GA2ox1 和GA2ox5。
方法
利用Gateway 技术法构建SlYABBY2b 基因超表达 和沉默载体,并通过农杆菌介导转化法获得转基 因植株。用酶联免疫吸附测定(ELISA)试剂盒 检测转基因植株中赤霉素的含量,用实时荧光定 量分析(qRT-PCR)赤霉素突变体中SlYABBY2b 基因表达水平和SlYABBY2b 转基因植株中赤霉素 相关基因的转录水平。
结论
本实验中赤霉素突变体中SlYABBY2b 基因表达量显示,赤霉素短缺导致番茄植株体内SlYABBY2b 基因的升高。ELISA 实验结果显示,SlYABBY2b 基因也能够反馈调控赤霉素的合成。qRT-PCR 结 果显示,SlYABBY2b 基因抑制GA20ox1 和GA3ox2 基因的表达,促进GA2ox1 和GA2ox5 基因的表 达。综上所述,研究结果明确了SlYABBY2b 基因 位于赤霉素信号转导的途径上,反馈调节赤霉素 的合成,感受外源赤霉素的信号,从而进一步调 控番茄心室的形成。
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Project supported by the China Agriculture Research System (No. CARS-25), the Program for Liaoning Innovative Research Team in University (No. LZ2015025), and the Program for Liaoning Key Laboratory (No. LZ2015064), China
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Li, H., Sun, Mh., Qi, Mf. et al. Alteration of SlYABBY2b gene expression impairs tomato ovary locule number and endogenous gibberellin content. J. Zhejiang Univ. Sci. B 19, 445–457 (2018). https://doi.org/10.1631/jzus.B1700238
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DOI: https://doi.org/10.1631/jzus.B1700238
Key words
- SlYABBY2b
- Gibberellin
- Gibberellin-deficient mutants
- Ovary locule number
- Transgenic plant
- Gene expression
- Tomato