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Aestuarium zhoushanense gen. nov., sp. nov., Isolated from the Tidal Flat

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Abstract

A gram-stain-negative, aerobic, ovoid or short rod-shaped, and non-motile strain, designed G7T was isolated from a tidal flat sample collected from the coast of East Sea in Zhoushan, China. Strain G7T grew at 4–40 °C and pH 6.0–9.0 (optimum, 28 °C and pH 7.5) and with 0–7% (w/v) NaCl (optimum, 1%). The predominant respiratory quinone was Q-10 and the major fatty acids (>10%) identified were C18:1 ω7c, C16:0 and summed feature 3 (C16:1 ω7c and/or C16:1 ω6c). The polar lipids of strain G7T consisted of phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, and four unidentified lipids. The genomic DNA G+C content was 56.7 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain G7T formed a distinct lineage belonging to the Roseobacter clade of the family Rhodobacteraceae. On the basis of morphological, physiological, and chemotaxonomic characteristics, together with the results of phylogenetic analysis, strain G7T is described as a novel species in a new genus, for which the name Aestuarium zhoushanense gen. nov., sp. nov. (type strain G7T = MCCC 1K03229T = KCTC 52584T) is proposed.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (Grant no. 31470005) and the Talent Startup Project of the Research Development Fund of Zhejiang Agricultural and Forestry University (2014FR074).

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Authors and Affiliations

  1. College of Life Sciences, Zhejiang University, Hangzhou, 310058, People’s Republic of China

    Xiao-Yun Yu, Xia Shen, Zhe Zhao & Min Wu

  2. College of Geography and Environmental Sciences, Zhejiang Normal University, Jinhua, 321004, People’s Republic of China

    Jing-Yu Zhai

  3. Ocean College, Zhejiang University, Zhoushan, 316000, People’s Republic of China

    Ge-Yi Fu

  4. School of Foresty and Biotechnology, Zhejiang Agriculture and Forestry University, Linan, 311300, People’s Republic of China

    Xin-Qi Zhang

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  1. Xiao-Yun Yu
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Correspondence to Min Wu or Xin-Qi Zhang.

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Additional information

The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequence and WGS whole-genome project of strain G7T are KX890235 and CP021114-CP021117, respectively. The Digital Protologue database TaxonNumber for strain G7T is TA00172.

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Yu, XY., Zhai, JY., Fu, GY. et al. Aestuarium zhoushanense gen. nov., sp. nov., Isolated from the Tidal Flat. Curr Microbiol 74, 1469–1476 (2017). https://doi.org/10.1007/s00284-017-1342-7

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  • DOI: https://doi.org/10.1007/s00284-017-1342-7

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